Thermo Fisher Scientific Human Vitamin D3 Receptor Recombinant Protein

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Baculovirus

Molecular weight

48 kDa

Class

Recombinant

Type

Protein

Purity

50% as determined by a SDS-PAGE gel stained with SimplyBlue™ SafeStain.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

proprietary buffer

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

This full-length human Vitamin D3 Receptor is purified from recombinant baculovirus-infected insect cells and is soluble and functionally active with an observed Kd for [3H]-1α, 25-dihydroxyvitamin D3 of 2 nM.

Vitamin D3 Receptor human recombinant protein is:

• Flexible-use in your in-house assay or as part of our PolarScreen™ Competitor assays for a complete assay solution
• Ready to use-active and native (untagged)
• Convenient-available in bulk sizes

Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.

Protein Form: Recombinant, Full Length

Target Information

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. The 1,25-dihydroxy-vitamin D3 receptor (VDR) belongs to the superfamily of steroid hormone receptors which includes estrogen, progesterone, glucocorticoid, androgen, and thyroid hormone receptors. Vitamin D modulates calcium and phosphorus homeostasis, bone remodeling, cell growth regulation, and differentiation. Studies have found VDR in the intestine, bone, kidney, epidermis, and cells of the endocrine immune system. Repression of T-cell proliferation and interleukin-2 (IL-2) gene expression, for example, occurs when VDR binds within the IL-2 enhancer. Formation of VDR/retinoic X receptor (RXR) heterodimers in the presence of intracellular 1,25(OH)2D3 has been shown to interfere with assembly of nuclear factor of activated T-cells (NFATp)/Fos/Jun/DNA complex and subsequent IL-2 gene transcription. The corresponding gene for the vitamin D receptor is NR1I1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.