Thermo Fisher Scientific B-Raf Polyclonal Antibody
Western blot, IHC, ICC/IF, Flow cytometry에 사용 가능한 Rabbit Polyclonal B-Raf 항체. Human에 반응하며, Protein A 및 항원 친화 크로마토그래피로 정제됨. MAPK/ERK 신호전달 연구 및 암 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:50 |
| Immunocytochemistry (ICC/IF) | 1:25 |
| Flow Cytometry (Flow) | 1:25 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to amino acids 424–453 of human B-RAF |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10979222 |
Product Specific Information
This antibody is predicted to react with chicken and mouse based on sequence homology.
Target Information
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer and is thought to play a role in the postsynaptic responses of hippocampal neurons. This protein regulates the MAP kinase/ERK signaling pathway, influencing cell division, differentiation, and secretion. Mutations in B-Raf are associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and lung adenocarcinoma. A pseudogene located on chromosome X has been identified for B-Raf.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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