Thermo Fisher Scientific B-Raf Polyclonal Antibody
Rabbit polyclonal antibody targeting human B-Raf protein. Suitable for WB, IHC, ICC/IF, and Flow Cytometry. Recognizes chicken and mouse by sequence homology. Supplied as 2 mg/mL liquid, unconjugated, for research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:50 |
| Immunocytochemistry (ICC/IF) | 1:10–1:50 |
| Flow Cytometry (Flow) | 1:10–1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide corresponding to amino acids 424–453 of human B-RAF |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10975898 |
Product Specific Information
This antibody is predicted to react with chicken and mouse based on sequence homology.
Target Information
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer and is thought to play a role in postsynaptic responses of hippocampal neurons. This protein regulates the MAP kinase/ERKs signaling pathway, influencing cell division, differentiation, and secretion. Mutations in B-Raf are associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and lung adenocarcinoma. A pseudogene located on chromosome X has been identified for B-Raf.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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