Thermo Fisher Scientific PLZF Monoclonal Antibody (9E12), APC, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 0.125 µg/test
• View 1 publication

Product Specifications

Additional Formats

• PE-Cyanine7
• Request custom conjugation

Product Specific Information

This 9E12 monoclonal antibody reacts with human and mouse promyelocytic leukemia zinc finger (PLZF), a member of the BTB-POZ family of transcription factors. Expression of this transcriptional repressor in immune cells differs between mice and humans.

• In mice: PLZF is highly expressed in immature CD1d-restricted NKT2 and NKT1 cells, and a subset of γδ (Vγ1.1⁺Vδ6.3⁺) T cells. It is also found in non-invariant CD1d-restricted T cells and non-CD1d-restricted innate T cells.
• In humans: PLZF is expressed in NK cells, γδ T cells, CD4⁺ and CD8⁺ T cells, MR1-specific mucosal-associated invariant T cells, and MHC Class II-restricted T cells developing via thymocyte–thymocyte interaction.
• PLZF is expressed by innate lymphoid cells and progenitor cells. It exists as a homodimer or complex with PLZP and is involved in NKT cell development, NK cell function, cellular quiescence, growth suppression, and homeostasis maintenance.
• It also inhibits gene expression induced by retinoic acid receptor.

This 9E12 antibody stains mouse thymocytes brighter than Mags.21F7.

Tested Application:
Intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00).
Recommended use ≤ 0.125 µg/test in a final volume of 100 µL. Cell number can range from 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal performance.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene encodes a zinc finger transcription factor of the Krueppel C2H2-type family, containing nine Kruppel-type zinc finger domains at the carboxyl terminus.
The protein is nuclear-localized, involved in cell cycle progression, and interacts with histone deacetylase. Aberrant gene rearrangements at this locus are associated with acute promyelocytic leukemia (APL). Alternate transcriptional splice variants have been identified.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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