Thermo Fisher Scientific Beta-2-adaptin Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunoprecipitation (IP)

• Tested Dilution: 2–10 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Based on 100% sequence identity, this antibody is predicted to react with Rat and Bovine

Target Information

Clathrin-mediated endocytosis is the pathway by which many receptors for nutrients and hormones are internalized to be recycled or down-regulated. During formation of clathrin-coated membranes, clathrin co-assembles with heterotetrameric molecules known as assembly polypeptides (APs) or adaptors, forming a layer of protein coat between the clathrin lattice and the membrane. There are two characterized adaptors, AP1 and AP2.

• AP1: Associated with clathrin-coated vesicles at the trans-Golgi network
• AP2: Associated with endocytic clathrin-coated vesicles at the plasma membrane; interacts with Shc and EGF receptor

AP2 is composed of four subunits: two separate ~100 kDa gene products (alpha and beta adaptin) and ~50 kDa and ~17 kDa subunits. There are two alpha-adaptin genes, alpha A and alpha C, which have tissue-specific expression patterns.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.