Thermo Fisher Scientific Human ICAM-2 Fc Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

CHO cells

Amino acid sequence

SDEKVFEVHV RPKKLAVEPK GSLEVNCSTT CNQPEVGGLE TSLDKILLDE QAQWKHYLVS NISHDTVLQC HFTCSGKQES MNSNVSVYQP PRQVILTLQP TLVAVGKSFT IECRVPTVEP LDSLTLFLFR GNETLHYETF GKAAPAPQEA TATFNSTADR EDGHRNFSCL AVLDLMSRGG NIFHKHSAPK MLEIYEPVSD SQGGPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGK

Tag

Fc-tag

Molecular weight

48.5 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by its ability to support the adhesion of PMA-stimulated HSB2 cells to plates pre-coated with ICAM-3 Fc. The ED50 for this effect is 1.0-5.0 ug/ml.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

PeproTech`s CHO-derived Recombinant Human ICAM-2 Fc is a glycosylated, disulfide-linked homodimer of 435 amino-acid-residues whose monomer consists of the 202-amino-acid extracellular portion of ICAM-2 fused to the 231-amino-acid length Fc portion of human IgG by two glycine residues. The calculated molecular weight of monomeric CHO cell-derived Recombinant Human ICAM-2 Fc is 48.5 kDa; however, due to glycosylation, it migrates at an apparent molecular weight of approximately 70-80 kDa by SDS-PAGE analysis under reducing conditions.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

ICAM-2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. ICAM-2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene. Diseases associated with ICAM2 include Colon Carcinoma In Situ.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.