Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), PE-Cyanine5, eBioscience

Applications

Tested Application

• Flow Cytometry (Flow)

Tested Dilution

• 5 µL (0.25 µg)/test

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Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488, 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV/Violet/Yellow series
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue/Red/Violet/Yellow series
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed on B cells during all developmental stages except terminally differentiated plasma cells. Follicular dendritic cells also express CD19. CD19 forms a multimolecular complex with CD21, CD81, Leu13, and MHC class II, which associates with the BCR to induce tyrosine phosphorylation, calcium flux, and B cell proliferation.
The SJ25C1 and HIB19 antibodies recognize overlapping epitopes.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested on normal human peripheral blood cells at 5 µL (0.25 µg)/test
• Light Sensitivity: Protect from light; tandem dye sensitive to photo-induced oxidation
• Fixation: Samples can be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in dark
• Excitation: 488–561 nm
• Emission: 667 nm
• Laser Compatibility: Blue, Green, Yellow-Green lasers
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on 100% of peripheral B cells and may also appear on certain myeloid leukemia cells. CD19 acts as a signal-amplifying coreceptor for the B cell receptor (BCR), regulating normal and malignant B cell proliferation. Mutations in CD19 can cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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