Thermo Fisher Scientific Phospho-ETS1 (Ser282, Ser285) Polyclonal Antibody
Phospho-ETS1 (Ser282, Ser285) 폴리클로날 항체로 인간과 랫트 시료에 반응. Western blot, IHC, ICC/IF, ChIP 등 다양한 응용 가능. Affinity chromatography로 정제되어 고순도 확보. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:50 | - |
| Immunocytochemistry (ICC/IF) | 1:250 | - |
| ChIP assay (ChIP) | 5 µg | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Chemically synthesized phosphopeptide derived from human ETS1 containing serine 282 and 285 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | Dulbecco’s PBS, pH 7.3, with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533578 |
Target Information
ETS1 (c-Ets-1; v-ets erythroblastosis virus E26 oncogene homolog 1 (avian); ETS-1; EWSR2; p54) is a 441 amino acid protein belonging to the DNA-binding ETS family.
It includes an N-terminal pointed domain, a central transactivation domain, and two auto-inhibitory domains flanked by a conserved C-terminal Ets domain.
ETS1 interacts with factors such as PEBP2alphaA and p300/CBP, mediating transactivation of Osteopontin, Presenilin-1, Stromelysin, and Collagenase.
Activated by Ras/MAPK signaling, ETS1 expression is upregulated in resting T-cells.
Its activity is modulated by AP-1, Sp-1, c-myb, and MafB.
ETS1 is activated by Sp100 in nuclear bodies and interacts with EAP1/Daxx to repress MMP1 and BCL2 transactivation.
It also interacts with STAT6 to modulate Socs-1 expression induced by IL-4 and with GATA3 to positively regulate Tax-1 dependent IL-5 expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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