Thermo Fisher Scientific AIF Polyclonal Antibody
AIF 단백질을 인식하는 Rabbit Polyclonal 항체로 Western Blot, IHC, IP에 사용 가능. Human 및 Mouse 시료에 반응하며, Affinity chromatography로 정제됨. PBS/sucrose buffer에 보관하며 -20°C에서 장기 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.2–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:600 |
| Immunoprecipitation (IP) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide directed towards the C-terminal region of human PDCD8 (aa 498–547) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 2% sucrose |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2884195 |
Product Specific Information
- Immunogen sequence:
VDSSLPTVGV FAKATAQDNP KSATEQSGTG IRSESETESE ASEITIPPST - Storage guidance:
For short-term use, store at 2–8°C up to 1 week.
For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles. - Predicted homology:
Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 93%; Rat: 100%; Sheep: 100%; Zebrafish: 79%
Target Information
Apoptosis Inducing Factor (AIF) induces chromatin condensation and DNA fragmentation. It is localized in mitochondria and translocates to the nucleus during apoptosis, promoting release of apoptogenic proteins such as cytochrome c and caspase-9. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, leading to severe mitochondrial encephalomyopathy. Multiple transcript variants and a pseudogene on chromosome 10 have been identified.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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