Thermo Fisher Scientific PODXL2 Polyclonal Antibody
PODXL2 단백질을 인식하는 Thermo Fisher Scientific의 염소 유래 폴리클로날 항체입니다. Western blot, ICC/IF, Flow cytometry에 사용 가능하며, 인간 시료에 반응합니다. 항원 친화 크로마토그래피로 정제되었으며, 보존제 없이 동결건조 형태로 제공됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL |
| Flow Cytometry (Flow) | 0.25 µg per million cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human Endoglycan/PODXL2 (Gly33–Thr500) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2576901 |
Product Specific Information
- In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human PODXL.
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Endotoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Podocalyxin-like protein 2 (PODXL2) is a CD34 family member expressed by podocytes, vascular endothelium, mesothelium, and a subset of hematopoietic progenitors. It is a major sialoprotein in the glycocalyx of glomerular podocytes. While its role in leukemic blasts is not fully understood, it is considered a potential prostate cancer tumor aggressiveness gene. PODXL2 complements CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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