Thermo Fisher Scientific ATG16L1 Polyclonal Antibody
ATG16L1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western Blot, IHC, ICC/IF, ELISA 등에 사용 가능. 인간, 마우스, 랫트 반응성. 액상 형태로 -20°C 보관, 고순도 Affinity Chromatography 정제.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 200–300 of human ATG16L1 (NP_1104305) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.32 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804798 |
Product Specific Information
- Immunogen sequence: QEANRLNAEN EKDSRRRQAR LQKELAEAAK EPLPVEQDDD IEVIVDETSD HTEETSPVRA ISRAATKRLS QPAGGLLDSI TNIFGRRSVS SFPVPQDNVD T
- Positive Samples: HT-29, MCF7, HeLa
- Cellular Location: Cytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane
Target Information
Autophagy related protein 16L (ATG16L1) is encoded by the ATG16L gene located on chromosome 2 in humans. It is also known as WD30 or IBD10 (Inflammatory Bowel Disease 10).
ATG12 and ATG5 conjugate and oligomerize with ATG16L to form an 800 kDa Atg16L complex, which facilitates lipidation of LC3 to phosphatidylethanolamine (PE). This process is a key marker for autophagy.
The ATG16L complex, together with ATG18 and ATG21, prevents premature cleavage of ATG8-PE by the cysteine protease Atg4 until autophagosome completion.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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