Thermo Fisher Scientific CD86 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:200

View 2 publications 2 publications

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 25-133 of human CD86 (NP_7870584) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD86,uniProtId:P42081-1,ncbiNodeId:9606,antigenRange:25-133,antigenLength:329,antigenImageFileName:PA5-88284_CD86_P42081-1_Rabbit.svg,antigenImageFileNamePDP:PA5-88284_CD86_P42081-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.19 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804800

Product Specific Information

Immunogen sequence: PLKIQAYFNE TADLPCQFAN SQNQSLSELV VFWQDQENLV LNEVYLGKEK FDSVHSKYMG RTSFDSDSWT LRLHNLQIKD KGLYQCIIHH KKPTGMIRIH QMNSELSVL

Positive Samples: THP-1; Cellular Location: Cell membrane, Single-pass type I membrane protein

Target Information

CD86 is one of two ligands (the other CD80) for CTLA4 and CD28. CD86 acts as costimulatory molecule in eliciting T-cell help during antigen presentation. Antigen presentation in the absence of sufficient co-stimulation involving CD86/CD80 can induce tolerance. CD80 appears to play a role distinct from CD80 in T helper cell differentiation. CD86 is a type I membrane protein that is a member of the immunoglobulin superfamily. The CD86 protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms of CD86. Additional transcript variants have been described for CD86, but their full-length sequences have not been determined. Diseases associated with CD86 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.