
Norgen Human Enterovirus (HEV) TaqMan RT-PCR Kit Dx
Human Enterovirus(HEV) 검출용 CE-IVDD 인증 TaqMan RT-PCR 키트. 양성·음성·PCR 억제 결과 확인 가능. Master Mix, Primer/Probe, Control 포함된 ready-to-use 구성. -20°C 보관, RNA 정제용으로 반복 동결 해동 최소화 필요.
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Norgen Human Enterovirus (HEV) TaqMan RT-PCR Kit Dx
SKU: DxTM39700
Features and Benefits
- Detection kits for HEV
- CE-IVDD marked in accordance with the European Commission Directive 98/79/EC
- Research version available
- Available in TaqMan format for analysis
Product Description
The Enteroviruses are a genus of the family Picornaviridae, consisting of small, positive-sense ssRNA viruses. Over 70 serotypes have been isolated from humans, including poliovirus, Coxsackie A/B, ECHO viruses, and others. Enteroviruses cause millions of infections annually, leading to various symptoms such as mild respiratory illness, hand-foot-mouth disease, rashes, conjunctivitis, meningitis, myocarditis, and neonatal infections.
Kit Configuration
HEV TaqMan RT-PCR Kit (24 reactions)
- Ready-to-use format with Master Mix and PCR control
- Specific Primer and Probe mix for the target pathogen
- Positive and negative controls included for validation
Storage Conditions
The kit is shipped on dry ice and should be stored at -20°C upon arrival. Avoid repeated freeze-thaw cycles (>3×) of Master Mix and Positive Control. If used intermittently, aliquot before freezing. Contact Norgen Biotek if any components arrive unfrozen or compromised.
Kit Components
| Component | Cat. DxTM39700 (24 rxns) |
|---|---|
| MDx TaqMan 2X RT-PCR Master Mix Dx | 550 µL |
| HEV Primer & Probe Mix Dx | 2 × 70 µL |
| HEV Positive Control Dx (200,000 copies/μL) | 50 µL |
| Nuclease-Free Water | 2 × 1.25 mL |
| Product Insert | 1 |
Supporting Data
Example Results
TaqMan PCR Kit – Figure 1 (Positive Result)
Both PCR signals above the baseline from FAM and HEX channels indicate successful amplification.
- 상단 그래프: Amplification (FAM channel, 파란색), Y축: RFU, X축: Cycles
- 하단 그래프: Amplification (HEX channel, 초록색), Y축: RFU, X축: Cycles
TaqMan PCR Kit – Figure 2 (Negative Result)
No target RNA detected in FAM channel; amplification signal from HEX indicates successful PCR.
- 상단 그래프: Amplification (FAM channel, 파란색), Y축: RFU, X축: Cycles
- 하단 그래프: Amplification (HEX channel, 초록색), Y축: RFU, X축: Cycles
TaqMan PCR Kit – Figure 3 (Inhibition Result)
No signals detected in both FAM and HEX channels. It is recommended to repeat sample preparation using the recommended RNA purification kit.
- 상단 그래프: Amplification (FAM channel, 파란색), Y축: RFU, X축: Cycles
- 하단 그래프: Amplification (HEX channel, 초록색), Y축: RFU, X축: Cycles
Documentation
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