Thermo Fisher Scientific Cyclin D3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide from the N-terminal of Human Cyclin D3 (1-100 aa), conjugated to Keyhole Limpet Haemocyanin (KLH) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Cyclin D3,uniProtId:P30281-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:292,antigenImageFileName:PA5-77799_Cyclin_D3_P30281-1_Rabbit.svg,antigenImageFileNamePDP:PA5-77799_Cyclin_D3_P30281-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2735696

Product Specific Information

A 1:1,000 dilution of PA5-77799 was sufficient for detection of Cyclin D3 in 15 µg of mouse brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.|Detects 45 kDa, due to post translational modifications.

Target Information

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. Diseases associated with CCND3 include Retinoblastoma and Plasma Cell Leukemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.