Thermo Fisher Scientific MCM4 Polyclonal Antibody
Thermo Fisher Scientific의 MCM4 Polyclonal Antibody는 인간 MCM4 단백질에 특이적인 항체로, WB, IHC, ICC/IF에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관. 연구용으로 DNA 복제 관련 단백질 분석에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human MCM4. Recombinant protein control fragment (Product #RP-102531) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789336 |
Product Specific Information
Immunogen sequence:
LPHTLLSRFD LIFLMLDPQD EAYDRRLAHH LVALYYQSEE QAEEELLDMA VLKDYIAYAH STIMPRLSEE ASQALIEAYV DMRKIGSSRG MVSAYPRQLE SLIRLAEAHA KVRLSNKVEA IDVEEAKRLH REALKQSAT
Target Information
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) essential for the initiation of eukaryotic genome replication. The hexameric MCM complex is a key component of the pre-replication complex (pre_RC), involved in replication fork formation and recruitment of other DNA replication-related proteins. The MCM complex (including MCM2, 4, 6, and 7) possesses DNA helicase activity and acts as a DNA unwinding enzyme. Phosphorylation by CDC2 kinase reduces helicase activity and chromatin binding. The gene is located on chromosome 8 adjacent to PRKDC/DNA-PK, a kinase involved in DNA double-strand break repair. Alternatively spliced transcript variants encoding the same protein have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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