Thermo Fisher Scientific Ki-67 Recombinant Rabbit Monoclonal Antibody (SP6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100

View 12 publications 12 publications

Immunohistochemistry (IHC)

-

View 646 publications 646 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

View 53 publications 53 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 11 publications 11 publications

Immunocytochemistry (ICC/IF)

1:250-1:500

View 73 publications 73 publications

Flow Cytometry (Flow)

1:1,000

View 7 publications 7 publications

Neutralization (Neu)

-

View 1 publication 1 publication

Functional Assay (Functional)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bovine, Cat, Chicken, Dog, Guinea pig, Hamster, Human, Mouse, Non-human primate, Pig, Rabbit, Rat, Rhesus monkey, Sheep

Host/Isotype

Rabbit / IgG

Expression System

proprietary

Class

Recombinant Monoclonal

Type

Antibody

Clone

SP6

Immunogen

Synthetic peptide within Human Ki67 aa 1200-1300 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Ki-67,uniProtId:P46013-1,ncbiNodeId:9606,antigenRange:1200-1300,antigenLength:3256,antigenImageFileName:MA5-14520_Ki-67_P46013-1_Rabbit.svg,antigenImageFileNamePDP:MA5-14520_Ki-67_P46013-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.031 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10979488

Product Specific Information

Staining of formalin-fixed tissues requires boiling tissue section in 10 mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Recommended positive controls:

IHC (P) - Human tonsil and testis tissue, common marmoset spleen tissue, rat esophagus, small intestine and liver tissue, mouse embryonic skin tissue

IHC (F) - Rat lymph node tissue, transgenic mouse spinal cord tissue

ICC/IF - HAP1 cells, human cardiac stem cells, HEp-2 cells, rat cardiomyocytes

Flow - HAP1 cells

Target Information

Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.