Thermo Fisher Scientific CD8 Monoclonal Antibody (SP16)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 39 publications 39 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

-

View 7 publications 7 publications

Flow Cytometry (Flow)

1:1,000

View 3 publications 3 publications

in situ PLA (PLA)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP16

Immunogen

Synthetic peptide sequence comprising the 13 C-terminal amino acids of the cytoplasmic domain of alpha chain of the CD8 molecule if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD8,uniProtId:P01732-1,ncbiNodeId:9606,antigenRange:223-235,antigenLength:235,antigenImageFileName:MA5-14548_CD8_P01732-1_Rabbit.svg,antigenImageFileNamePDP:MA5-14548_CD8_P01732-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.022 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984334

Product Specific Information

MA5-14548 targets CD8 in IHC (P), and FACS applications and shows reactivity with Human samples.

The MA5-14548 immunogen is synthetic peptide sequence comprising the 13 C-terminal amino acids of the cytoplasmic domain of alpha chain of the CD8 molecule.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.