Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), Super Bright 436, eBioscience
Mouse CD3e 인식하는 Armenian hamster 유래 단클론 항체로, Super Bright 436 형광체 결합. Flow cytometry에 최적화되어 있으며, T세포 표지 및 활성화 연구에 활용. 405 nm 레이저로 여기, 436 nm에서 방출. 4°C 보관, 동결 금지.
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1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), Super Bright 436, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 7 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1.0 µg/Test | View 41 publications |
| ELISA | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 145-2C11 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2784796 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 496 / 563 / 615 / 661 / 737
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue / Red / Yellow series
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 600 / 780
- Request custom conjugation
Product Specific Information
The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. CD3e, together with other CD3 subunits (gamma and delta), is essential for proper assembly and surface expression of the TCR complex. CD3 is expressed developmentally in thymocytes and on all mature T cells. Binding of 145-2C11 to TCR initiates intracellular signaling leading to activation, proliferation, or apoptosis depending on conditions.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes, recommended ≤1.0 µg/test (in 100 µL final volume). Optimize antibody titration per assay.
Fluorochrome Information:
Super Bright 436 is excited by the 405 nm violet laser and emits at 436 nm. Recommended detection filter: 450/50 bandpass (or equivalent). Ensure instrument compatibility.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. It includes subunits CD3γ, CD3δ, CD3ε, and CD3ζ, encoded by genes on chromosome 11. These subunits have cytoplasmic tails associated with signal transduction molecules via tyrosine-based motifs.
CD3 is expressed on 68–82% of normal peripheral blood lymphocytes, 65–85% of thymocytes, and Purkinje cells in the cerebellum, but not on B or NK cells. Reduced T lymphocytes may be observed in some autoimmune diseases. Defects in CD3 genes are linked to CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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