Thermo Fisher Scientific SARS-CoV-2 Spike Protein (RBD) (aa330-524), His Tag Recombinant Protein
SARS-CoV-2 Spike Protein RBD (aa330-524) 재조합 단백질로, His-tag 부착. ELISA, Western blot 등 다양한 연구용으로 사용 가능. >95% 순도, E. coli 발현 시스템 기반. 바이러스 단백질 결합 및 항체 연구에 적합.
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Applications
- Western Blot (WB): Assay-dependent
- ELISA (ELISA): Assay-dependent
- Control (Ctrl): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Virus |
| Expression System | E. coli |
| Amino Acid Sequence | SARS-CoV-2 Spike Protein (s-RBD) contains amino acids 330–524 of the SARS-CoV-2 Spike protein with a His-tag |
| Tag | His-tag |
| Molecular Weight | 27 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | >95% by SDS-PAGE under reducing conditions |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not determined |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
Product Specific Information
RP-87674 has been tested in indirect ELISA.
Reconstitution: Centrifuge the vial briefly (~30 seconds) before opening to bring contents to the bottom. Reconstitute with 400 µL of deionized sterile water to obtain a final concentration of 0.5 mg/mL. Let the tube sit on ice for ~30 minutes with gentle pipetting periodically.
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), also known as 2019-nCoV, causes illnesses ranging from mild cold symptoms to severe respiratory distress and death.
The CoV Spike (S) protein plays key roles in viral attachment, fusion, and entry, serving as a target for antibody, entry inhibitor, and vaccine development.
The receptor-binding domain (RBD, S-RBD) of the Spike protein binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 compared to SARS-CoV RBD.
The S1 subunit contains the RBD that specifically interacts with ACE2 receptors on target cells.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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