Thermo Fisher Scientific SARS-CoV-2 Spike Protein (RBD) (aa330-524), His Tag Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Virus

Expression System

E. coli

Amino acid sequence

SARS-CoV-2 Spike Protein (s-RBD) contains amino acids 330-524 of the SARS-CoV-2 Spike protein with a His-tag

Tag

His-tag

Molecular weight

27 kDa

Class

Recombinant

Type

Protein

Purity

>95% by SDS-PAGE under reducing conditions

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

purified

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

RP-87674 has been tested in indirect ELISA.

Reconstitution: It is recommend that the vial be centrifuged briefly (~30 seconds) before opening the tube in order to bring the contents to the bottom. Then, reconstitute the protein with 400 µL of deionized sterile water to obtain a final concentration of 0.5 mg/mL. Let the tube sit on ice for ~30 minutes with gentle pipetting periodically.

Target Information

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.