Thermo Fisher Scientific ATG16L1 Polyclonal Antibody
Rabbit polyclonal antibody against ATG16L1 protein for WB and IHC applications. Reacts with human, mouse, and rat. Lyophilized form, reconstitutable in sterile water. Suitable for autophagy research and ATG16L complex studies.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:100–1:2,000 | – |
| Immunohistochemistry (IHC) | 1:100–1:2,000 | – |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from amino acid region 170–210 of mouse ATG16L1 conjugated to blue carrier protein. The peptide is homologous in rat and human. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4 °C for short term. For long-term storage, store at −20 °C and avoid freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 150 µL of sterile water.
- Centrifuge to remove any insoluble material.
- Specificity: ATG16L1.
Target Information
Autophagy-related protein 16L (encoded by the ATG16L gene) is located on chromosome 2 in humans and also known as WD30 or IBD10 (Inflammatory Bowel Disease 10).
ATG12 and ATG5 conjugate and oligomerize with ATG16L to form an ~800 kDa complex (Atg16L complex), which facilitates lipidation of LC3 to phosphatidylethanolamine (PE) — a key marker for autophagy.
This complex, along with ATG18 and ATG21, prevents premature cleavage of ATG8-PE by the cysteine protease Atg4 until the autophagosome is complete.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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