Thermo Fisher Scientific ATG16L1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:2,000

-

Immunohistochemistry (IHC)

1:100-1:2,000

-

Miscellaneous PubMed (Misc)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Not Applicable

Host/Isotype

Rabbit / Ig

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from n-terminal region of human autophagy-related protein 16-1 (ATG16L1, APG16L) conjugated to an immunogenic carrier protein has been used as the antigen if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ATG16L1,uniProtId:Q676U5-1,ncbiNodeId:9606,antigenRange:1,antigenLength:607,antigenImageFileName:OSA00129W-150UL_ATG16L1_Q676U5-1_Rabbit.svg,antigenImageFileNamePDP:OSA00129W-150UL_ATG16L1_Q676U5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.

Specificity of this antibody: ATG16L1.

Target Information

Autophagy related protein 16 L is encoded by the ATG16L gene and is located on chromosome 2 in humans. It is also known as WD30 or IBD10 (Inflammatory Bowel Disease 10). ATG 12 and ATG5 conjugate with each other and undergoes homo oligomerisation with ATG16L to form an 800 kDa complex called the Atg16L complex. This complex facilitates coupling or lipidation of LC3 to phosphatidylethanolamine (PE) and this ubiquitin-like conjugation system is a widely used marker for autophagy. However, the ATG 16L complex along with other ATGs like ATG 18 and ATG 21 prevent the premature cleavage of ATG8-PE by the cysteine protease, Atg4 until the autophagosome is deemed complete.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.