Thermo Fisher Scientific Apolipoprotein B /APOB Monoclonal Antibody (APOB, 4333)

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
• Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.

Target Information

Apolipoprotein B (apo B) in human plasma is a major protein of low-density lipoproteins (LDL) with a molecular mass of approximately 260–500 kDa.
Apolipoprotein B binds to specific receptors on cell membranes and is involved in the removal of LDL and very low-density lipoprotein (VLDL) cholesterol from circulation.

LDL is the carrier protein for cholesterol in the blood and mediates uptake and clearance of cholesterol via receptor binding.
Oxidized LDL is found in atherosclerotic lesions and is recognized by macrophages through scavenger receptors (A and CD36).
Elevated levels of Apolipoprotein B are associated with premature atherosclerosis.

Apolipoprotein B occurs as two main isoforms, apoB-48 and apoB-100, encoded by a single gene.
The shorter apoB-48 is produced by RNA editing of apoB-100 transcript, introducing a stop codon at residue 2180.
Mutations in the Apolipoprotein B gene or regulatory regions can cause disorders such as hypobetalipoproteinemia and hypercholesterolemia due to ligand-defective Apolipoprotein B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.