Thermo Fisher Scientific Tubulin-b Monoclonal Antibody (ABM323)
Beta-tubulin 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 세포질 위치 검출에 적합하며, 연구용으로 미세소관 관련 단백질 분석에 활용.
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Thermo Fisher Scientific Tubulin-b Monoclonal Antibody (ABM323)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ABM323 |
| Immunogen | A synthetic peptide derived from internal sequence of Tubulin beta protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Lung tissue, HeLa cells
- Cellular location: Cytoplasmic
Target Information
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance, and maintenance. Mutations in the beta tubulin gene are associated with congenital fibrosis of the extraocular muscles type 3.
Beta-III tubulin is also detected in Sertoli cells and transiently in non-neuronal embryonic tissues. Tubulin can be modified by glutamylation at its C-terminal glutamate residues, though the functional significance is unclear.
Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression, which inhibits incorporation into microtubules. Microtubules, composed of alpha and beta tubulin dimers (~55 kDa each), are essential for mitotic chromosome segregation, intracellular transport, ciliary and flagellar movement, and cytoskeletal structure.
Because beta-tubulin is ubiquitously expressed in eukaryotic cells, it is frequently used as a loading control in protein detection assays such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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