Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Alexa Fluor 700, eBioscience

Applications

• Tested Application: Flow Cytometry (Flow)
• Recommended Dilution: 5 µL (0.5 µg)/test
• Publications: View 24 publications

Product Specifications

Available Conjugate Formats

• Unconjugated
• Alexa Fluor 532, 561, 660
• APC, APC-eFluor 780
• Biotin
• Brilliant UV/Violet/Blue/Yellow/Red series
• eFluor 450, 506
• FITC
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• Super Bright series
• NovaFluor series
• Request custom conjugation

Product Specific Information

The UCHT1 monoclonal antibody reacts with human CD3ε, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits (γ and δ), the ε chain is required for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells.

Crosslinking of TCR via immobilized UCHT1 initiates intracellular signaling, resulting in cellular activation and proliferation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested using normal human peripheral blood cells
• Recommended Usage: 5 µL (0.5 µg) per test in 100 µL final volume (10⁵–10⁸ cells/test)
• Fluorochrome Details: Alexa Fluor® 700 emits at 723 nm, excited with red laser (633 nm). Recommended optical setup: 685 LP mirror, 710/20 filter.
• Excitation/Emission: 633–647 nm / 723 nm
• Laser: Red Laser
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

The CD3 subunit complex is essential for transducing antigen-recognition signals into T cells and regulating TCR surface expression. CD3 subunits (γ, δ, ε, ζ) are structurally related members of the immunoglobulin superfamily encoded on human chromosome 11.

CD3 plays roles in TCR-induced growth arrest, survival, and proliferation. It is expressed on:

• 68–82% of normal peripheral blood lymphocytes
• 65–85% of thymocytes
• Purkinje cells in the cerebellum

It is not expressed on B or NK cells. Defects in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지