Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), Alexa Fluor 700, eBioscience
인간 CD3ε를 인식하는 UCHT1 단클론 항체로, Alexa Fluor 700으로 표지되어 유세포분석(Flow Cytometry)에 최적화되었습니다. T 세포 표면 CD3 복합체 분석에 사용되며, 4°C 암소 보관이 필요합니다. 연구용으로만 사용 가능합니다.
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Applications
- Tested Application: Flow Cytometry (Flow)
- Recommended Dilution: 5 µL (0.5 µg)/test
- Publications: View 24 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 700, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UCHT1 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation/Emission Max | 696/719 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10597906 |
Available Conjugate Formats
- Unconjugated
- Alexa Fluor 532, 561, 660
- APC, APC-eFluor 780
- Biotin
- Brilliant UV/Violet/Blue/Yellow/Red series
- eFluor 450, 506
- FITC
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- Super Bright series
- NovaFluor series
- Request custom conjugation
Product Specific Information
The UCHT1 monoclonal antibody reacts with human CD3ε, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits (γ and δ), the ε chain is required for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells.
Crosslinking of TCR via immobilized UCHT1 initiates intracellular signaling, resulting in cellular activation and proliferation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and tested using normal human peripheral blood cells
- Recommended Usage: 5 µL (0.5 µg) per test in 100 µL final volume (10⁵–10⁸ cells/test)
- Fluorochrome Details: Alexa Fluor® 700 emits at 723 nm, excited with red laser (633 nm). Recommended optical setup: 685 LP mirror, 710/20 filter.
- Excitation/Emission: 633–647 nm / 723 nm
- Laser: Red Laser
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
The CD3 subunit complex is essential for transducing antigen-recognition signals into T cells and regulating TCR surface expression. CD3 subunits (γ, δ, ε, ζ) are structurally related members of the immunoglobulin superfamily encoded on human chromosome 11.
CD3 plays roles in TCR-induced growth arrest, survival, and proliferation. It is expressed on:
- 68–82% of normal peripheral blood lymphocytes
- 65–85% of thymocytes
- Purkinje cells in the cerebellum
It is not expressed on B or NK cells. Defects in CD3 genes are associated with CD3 immunodeficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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