Thermo Fisher Scientific HSF1 Recombinant Rabbit Monoclonal Antibody (23GB2715)

Applications and Tested Dilutions

Product Specifications

Target Information

All organisms respond to elevated temperatures and various environmental stresses by synthesizing heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are composed of three repeats of a 5-nucleotide {nGAAn} module arranged in alternating orientation and are present upstream of all heat shock genes.

Although HSEs are highly conserved among species, HSF proteins from yeast, Drosophila, and human differ in molecular weight and show limited immunological cross-reactivity. In humans, two HSFs—HSF1 and HSF2—have been identified, sharing 38% sequence identity and binding to the same HSEs. HSF1 responds to classical stress signals such as heat, heavy metals, and oxidative reagents, while HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells.

HSF1 exists constitutively in the cytoplasm and nucleus of unstressed cells as a monomer lacking DNA binding activity. Upon stress, HSF1 transitions into a nuclear-localized trimeric form that binds DNA. Phosphorylation of HSF1 is required for maximal transcription of heat shock genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.