
Thermo Fisher Scientific Rat IgG1 kappa Isotype Control (eBRG1), Pacific Orange, eBioscience
Flow cytometry용 Rat IgG1 kappa 동종대조 항체로, Pacific Orange 형광체 결합. 비특이적 결합 평가용 음성 대조에 적합. Violet laser(405 nm)로 여기되며 551 nm에서 방출. 4°C 보관, 동결 금지.
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Applications
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rat / IgG1, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBRG1 |
| Conjugate | Pacific Orange™ |
| Excitation / Emission Max | 400 / 551 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2815354 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 647
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
- eFluor 450 / 506 / 570 / 615 / 660
- FITC
- Functional Grade
- PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5 / PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Custom conjugation available upon request
Product Specific Information
The monoclonal rat IgG1, kappa (clone eBRG1) is useful as an isotype control immunoglobulin.
This antibody has been reported for use in flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells.
Isotype control should be used at the same concentration as the experimental antibody.
Pacific Orange emits at 551 nm and is excited with the violet laser line (405 nm). Ensure that your instrument supports detection of this fluorochrome.
Excitation: 405 nm
Emission: 551 nm
Laser: Violet Laser
Target Information
Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies commonly used in research and diagnostics. These antibodies, produced by rats in response to antigens, can be purified from serum or hybridoma supernatants.
Rat IgG1 is often used as a negative control in experiments, as it does not bind to many mammalian antigens. It is applicable in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens.
Gene targeting in rats enables generation of animal models for studying human diseases by knocking out, inserting, or modifying specific genes to investigate their roles in disease mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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