Thermo Fisher Scientific FBXW7 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:1,000

Product Specifications

Product Specific Information

• PA1-4253 detects the F-box hCdc4 (69 kDa isoform) protein in human samples.
• Successfully used in Western blot procedures.
• Immunizing peptide corresponds to the N-terminus of the 69 kDa isoform of the hCdc4 protein.
• Maintain at 4°C for 3 months or at -20°C for longer periods. Avoid freeze/thaw cycles.

Target Information

F-Box and WD Repeat Domain Containing 7 (FBXW7) is a member of the F-box protein family characterized by an approximately 40 amino acid F-box motif.
F-box proteins form part of the SCF (SKP1-cullin-F-box) ubiquitin ligase complex, functioning in phosphorylation-dependent ubiquitination.
They are divided into three classes:

• Fbws: containing WD-40 domains
• Fbls: containing leucine-rich repeats
• Fbxs: containing other interaction modules or no recognizable motifs

FBXW7 (formerly FBX30) belongs to the Fbws class and contains 7 tandem WD40 repeats.
It binds directly to cyclin E and likely targets it for ubiquitin-mediated degradation.
Mutations in this gene have been observed in ovarian and breast cancer cell lines, suggesting its role in cancer pathogenesis.
Multiple transcript variants encoding different isoforms have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.