Thermo Fisher Scientific PASK Monoclonal Antibody (OTI8G9), TrueMAB

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Immunocytochemistry (ICC/IF)

1:100

-

Flow Cytometry (Flow)

1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI8G9

Immunogen

Full length human recombinant protein of human PASK produced in HEK293T cell. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PASK,uniProtId:Q96RG2-1,ncbiNodeId:9606,antigenRange:1-1323,antigenLength:1323,antigenImageFileName:CF500826_PASK_Q96RG2-1_House_mouse.svg,antigenImageFileNamePDP:CF500826_PASK_Q96RG2-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

PASK is an evolutionarily conserved protein present in yeast, flies, and mammals. PAS domains regulate the function of many intracellular signaling pathways in response to both extrinsic and intrinsic stimuli. It is a conserved gene product found in yeast as well as mammals. PASKIN contains two domains (PAS A and PAS B) as well as a serine/threonine kinase domain related to AMP kinases. PASKIN activity leads to decreased carbohydrate storage, as well as increased protein synthesis. PASKIN-dependent phosphorylation inhibits the activity of mammalian glycogen synthase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.