Thermo Fisher Scientific Lactoferrin Monoclonal Antibody (4C5), PE

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse

Class

Monoclonal

Type

Antibody

Clone

4C5

Immunogen

Human Lactoferrin

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536532

Product Specific Information

R-phycoerythrin (PE) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients.

Target Information

Lactoferrin (LF) is an iron binding glycoprotein. LF consists of a single polypeptide chain (approximately 80 kDa) folded into two structurally homologous lobes, each of which can reversibly bind one ferric ion (Fe3+). LF is found in external fluids, including milk and mucosal secretions, and is a prominent component of secondary granuals of neutrophils. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. LTF acts as a major first line defense against microbial infections, partly due to its ability to efficiently bind to and hence remove Fe3+ from the environment, but studies have also shown that LTF possesses bactericidal properties and proteolytic activity, capable of cleaving arginine-rich sequences within microbial proteins. Three isoforms of LTF have been identified: LTF-alpha, LTF-beta and LTF-gamma and receptors for LTF are expressed in the gastrointestinal tract, by macrophages, lymphocytes, neutrophils and platelets and also by some bacteria.

Analyte Specific Reagent