Thermo Fisher Scientific Amphiphysin Polyclonal Antibody
Human Amphiphysin 단백질에 특이적인 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF 분석에 최적화되어 있습니다. 항원 친화 크로마토그래피로 정제되었으며, PBS buffer에 40% glycerol 포함. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:2,500 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human AMPH. Recombinant protein control fragment (Product #RP-92625) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2789858 |
Product Specific Information
Immunogen sequence:
PGFLYKVETL HDFEAANSDE LTLQRGDVVL VVPSDSEADQ DAGWLVGVKE SDWLQYRDLA TYKGLFPENF TRRL
Target Information
Amphiphysins, members of the BAR (Bin-Amphiphysin-Rvsp) protein superfamily, play a key role in clathrin-mediated endocytosis of synaptic vesicles (SVs).
All members share a conserved N-terminal BAR domain and a C-terminal Src homology (SH3) domain.
Two isoforms of amphiphysin act as a heterodimer and interact via the SH3 domain with dynamin, synaptojanin, and clathrin.
These complexes are involved in synaptic vesicle recycling at nerve terminals.
Amphiphysins were also identified as the dominant autoantigen in paraneoplastic Stiff-Man Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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