Thermo Fisher Scientific Phospho-MDM2 (Ser186, Ser188) Polyclonal Antibody

Applications

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:200
• Suggested Positive Control: HeLa cells

Product Specifications

Product Specific Information

A suggested positive control for ICC/IF is HeLa cells.

Target Information

MDM2 is a ubiquitin ligase for p53 and plays a central role in the regulation of p53 stability. It is located on chromosome 12q. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 enhances its interaction with p300, promoting MDM2-mediated ubiquitination and degradation of p53. Phosphorylation also prevents MDM2 binding to p19ARF, further increasing p53 degradation. Overexpression of MDM2 can inactivate p53, reducing its tumor suppressor function. MDM2 also interacts with retinoblastoma 1 and ribosomal protein L5, influencing cell cycle, apoptosis, and tumorigenesis. It is overexpressed in various human cancers, including soft tissue carcinoma and breast cancer. More than 40 alternatively spliced transcript variants of MDM2 have been identified in tumor and normal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.