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Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Alexa Fluor 561, eBioscience
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Thermo Fisher Scientific

Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Alexa Fluor 561, eBioscience

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마우스 CD25(IL-2Rα) 검출용 Alexa Fluor 561 결합 단클론 항체로, Flow Cytometry에 최적화됨. 0.25 µg/test 권장 사용량. Rat IgG1, λ 아이소타입이며 4°C 암소 보관. IL-2 수용체 결합 억제 및 림프구 활성화 연구에 적합.

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Thermo Fisher Scientific
카테고리
Monoclonal Antibodies
판매단위
pk

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Thermo Fisher Scientific 505-0251-80 CD25 Monoclonal Antibody (PC61.5), Alexa Fluor 561, eBioscience 25 ug pk판매 단위 pk
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334,400원VAT 포함 367,840원
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Thermo Fisher Scientific · Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Alexa Fluor 561, eBioscience

Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Alexa Fluor 561, eBioscience

Applications

  • Flow Cytometry (Flow)
    Tested Dilution: 0.25 µg/test

Product Specifications

항목 내용
Species Reactivity Mouse
Host / Isotype Rat / IgG1, lambda
Recommended Isotype Control Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 561
Class Monoclonal
Type Antibody
Clone PC61.5
Conjugate Alexa Fluor™ 561
Excitation / Emission Max 556 / 569 nm
Form Liquid
Concentration 0.2 mg/mL
Purification Affinity chromatography
Storage buffer PBS, pH 7.2
Contains 0.09% sodium azide
Storage conditions 4°C, store in dark, DO NOT FREEZE
Shipping conditions Ambient (domestic); Wet ice (international)
RRID AB_2896196

Additional Formats

  • Unconjugated
  • Alexa Fluor 488, 532, 647, 660, 700
  • APC, APC-eFluor 780
  • Biotin
  • Brilliant UV 563, 661, 737, 805
  • Brilliant Violet 421, 650, 711, 786
  • eFluor 450, 506
  • FITC
  • Functional Grade
  • NovaFluor Blue 585, 610-70S, 660-120S, 760
  • Pacific Orange
  • PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
  • PerCP-Cyanine5.5, PerCP-eFluor 710
  • Super Bright 436, 600, 645, 702, 780
  • Request custom conjugation available

Product Specific Information

The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early T/B progenitors and activated mature lymphocytes. It binds IL-2 with low affinity alone but forms a high-affinity receptor when associated with CD122 and CD132.
Binding of IL-2 to its receptor is inhibited by PC61.5 antibody, which plays a role in lymphocyte differentiation and activation.

  • Applications Reported: Flow cytometric analysis
  • Applications Tested: Mouse splenocytes, ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
    Titration recommended for optimal performance.
  • Alexa Fluor 561 emits at 575 nm; suitable for spectral cytometers multiplexed with PE and PE-eFluor 610.
  • Excitation: 558 nm
    Emission: 575 nm
    Laser: Yellow-Green Laser

Target Information

CD25 (IL2RA) encodes the IL-2 receptor alpha chain, a cytokine receptor involved in T and B cell proliferation. The high-affinity IL-2 receptor complex includes IL2RA, IL2RB, and IL2RG. IL2 primarily produced by mature T cells acts as a growth and differentiation factor and regulates cell death.
Mutations in IL2RA are linked to immune dysregulation disorders such as ulcerative colitis-like disease in mice.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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