Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Alexa Fluor 561, eBioscience
마우스 CD25(IL-2Rα) 검출용 Alexa Fluor 561 결합 단클론 항체로, Flow Cytometry에 최적화됨. 0.25 µg/test 권장 사용량. Rat IgG1, λ 아이소타입이며 4°C 암소 보관. IL-2 수용체 결합 억제 및 림프구 활성화 연구에 적합.
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Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Alexa Fluor 561, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG1, lambda |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 561 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PC61.5 |
| Conjugate | Alexa Fluor™ 561 |
| Excitation / Emission Max | 556 / 569 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896196 |
Additional Formats
- Unconjugated
- Alexa Fluor 488, 532, 647, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 563, 661, 737, 805
- Brilliant Violet 421, 650, 711, 786
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue 585, 610-70S, 660-120S, 760
- Pacific Orange
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation available
Product Specific Information
The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early T/B progenitors and activated mature lymphocytes. It binds IL-2 with low affinity alone but forms a high-affinity receptor when associated with CD122 and CD132.
Binding of IL-2 to its receptor is inhibited by PC61.5 antibody, which plays a role in lymphocyte differentiation and activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Mouse splenocytes, ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
Titration recommended for optimal performance. - Alexa Fluor 561 emits at 575 nm; suitable for spectral cytometers multiplexed with PE and PE-eFluor 610.
- Excitation: 558 nm
Emission: 575 nm
Laser: Yellow-Green Laser
Target Information
CD25 (IL2RA) encodes the IL-2 receptor alpha chain, a cytokine receptor involved in T and B cell proliferation. The high-affinity IL-2 receptor complex includes IL2RA, IL2RB, and IL2RG. IL2 primarily produced by mature T cells acts as a growth and differentiation factor and regulates cell death.
Mutations in IL2RA are linked to immune dysregulation disorders such as ulcerative colitis-like disease in mice.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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