Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Alexa Fluor 561, eBioscience
마우스 CD19에 반응하는 eBio1D3 (1D3) 단클론 항체로, Alexa Fluor 561 형광체가 결합되어 있습니다. Flow cytometry용으로 최적화되었으며, 0.25 µg/test 권장. B세포 발달 단계 분석에 적합하며, 특정 마우스 종에서 비특이적 염색 주의 필요.
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Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Alexa Fluor 561, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 561, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio1D3 (1D3) |
| Conjugate | Alexa Fluor™ 561 |
| Excitation / Emission Max | 556 / 569 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896192 |
Available Formats
- Unconjugated
- Alexa Fluor 660
- Alexa Fluor 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV / Violet / Yellow / NovaFluor series
- PE, PE-Cyanine, PerCP-Cyanine
- Super Bright series
- Request custom conjugation
Product Specific Information
The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all stages of development except plasma cells. Follicular dendritic cells also express CD19. CD19 forms a complex with CD21, CD81, and MHC class II associated with the BCR, mediating signaling that induces tyrosine phosphorylation, calcium flux, and B cell proliferation.
- Applications Reported: Flow cytometric analysis.
- Applications Tested: Tested by flow cytometric analysis of mouse splenocytes; ≤ 0.25 µg/test recommended.
A test is defined as antibody amount staining cells in 100 µL final volume (10^5–10^8 cells/test).
Careful titration recommended for optimal performance. - Alexa Fluor 561 emits at 575 nm and is suitable for spectral cytometers; multiplexable with PE and PE-eFluor 610.
- Excitation: 558 nm
- Emission: 575 nm
- Laser: Yellow-Green Laser
Note: Internal testing shows Alexa Fluor 561 non-specifically stains B cells in Swiss Webster and SJL mice. Non-specific staining not observed in BALB/c or C57BL/6 mice.
Target Information
CD19 is a member of the immunoglobulin superfamily with two Ig-like domains, expressed on 100% of peripheral B cells. It may also be found on myeloid leukemia cells. CD19 serves as a critical regulator of normal and malignant B cell proliferation, functioning as a co-receptor with BCR to lower activation thresholds. Mutations cause hypogammaglobulinemia; overexpression leads to B cell hyperactivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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