Thermo Fisher Scientific RPL14 Polyclonal Antibody
Rabbit polyclonal antibody against human RPL14. Validated for Western blot and immunocytochemistry. Recognizes ribosomal protein L14 involved in protein synthesis. Supplied as liquid, 0.39 mg/mL, store at -20°C. For research use only.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein corresponding to amino acids 1–215 of human RPL14 (NP_003964.3) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.39 mg/mL |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2805418 |
Product Specific Information
Immunogen sequence:
MVFRRFVEVG RVAYVSFGPH AGKLVAIVDV IDQNRALVDG PCTQVRRQAM PFKCMQLTDF ILKFPHSAHQ KYVRQAWQKA DINTKWAATR WAKKIEARER KAKMTDFDRF KVMKAKKMRN RIIKNEVKKL QKAALLKASP KKAPGTKGTA AAAAAAAAAK VPAKKITAAS KKAPAQKVPA QKATGQKAAP APKAQKGQKA PAQKAPAPKA SGKKA
Positive Samples: HL-60, HeLa, 293T, Jurkat, HepG2, Mouse spleen, Mouse ovary, Rat spleen, Rat kidney
Target Information
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. These subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins.
This gene encodes a ribosomal protein that is a component of the 60S subunit and belongs to the L14E family. It contains a basic region-leucine zipper (bZIP)-like domain and is located in the cytoplasm.
The gene includes a polymorphic trinucleotide (GCT) repeat tract, resulting in a stretch of alanine residues. Multiple transcript variants exist but encode the same protein. As typical for ribosomal protein genes, multiple processed pseudogenes are dispersed throughout the genome.
References:
- Aoki M., Diabetes 45:157–164 (1996)
- Tanaka M., Biochem. Biophys. Res. Commun. 243:531–537 (1998)
- Yoshihama M., Genome Res. 12:379–390 (2002)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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