Thermo Fisher Scientific IL-31 Monoclonal Antibody (31SNEZE), eFluor 660, eBioscience
인간 IL-31을 인식하는 단클론 항체로, eFluor 660 형광 표지되어 유세포분석(Flow Cytometry)에 적합합니다. Th2 세포 및 비만세포의 IL-31 발현 연구에 활용됩니다. 고순도 정제 및 안정한 보관 용액 구성으로 재현성 높은 결과를 제공합니다.
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Applications and Tested Dilution
- Application: Flow Cytometry (Intracellular Staining)
- Tested Dilution: 5 µL (0.125 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 31SNEZE |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in the dark, DO NOT FREEZE! |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637151 |
Product Specific Information
Description:
The 31SNEZE monoclonal antibody reacts with human IL-31, a member of the IL-6 cytokine family. IL-31 is expressed by CD4⁺ T cells (particularly Th2 phenotype) and mast cells. The IL-31 receptor is a heterodimer composed of IL-31RA and OSMRβ subunits, expressed by macrophages, monocytes, eosinophils, keratinocytes, and epithelial cells. IL-31 receptor signaling activates STAT3 and promotes release of proinflammatory cytokines and chemokines such as IL-1β, IL-6, IL-8, CXCL1, and CCL2. Overexpression of IL-31 is correlated with allergic asthma, rhinitis, and atopic dermatitis severity.
Applications Reported:
Validated for intracellular staining followed by flow cytometric analysis.
Applications Tested:
Pre-titrated for use in intracellular staining and flow cytometry of human peripheral blood lymphocytes. Recommended usage: 5 µL (0.125 µg) per 100 µL test volume. Cell number may range from 10⁵ to 10⁸ cells/test.
Fluorochrome Information:
eFluor® 660 replaces Alexa Fluor® 647, emitting at 659 nm and excited by the red laser (633 nm). Ensure instrument compatibility.
- Excitation: 633–647 nm
- Emission: 668 nm
- Laser: Red Laser
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Interleukin-31 (IL-31) is a T-cell cytokine related to IL-6 family cytokines, preferentially produced by Th2 cells. IL-31 acts through a receptor complex of IL-31RA and oncostatin M receptor (OSMR). This interaction activates JAK/STAT and AKT signaling pathways. IL-31 receptor expression is constitutive in epithelial cells and inducible in activated monocytes. Overexpression in transgenic mice induces pruritus, alopecia, and skin lesions, implicating IL-31 in atopic dermatitis and as a potential antipruritic drug target.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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