Thermo Fisher Scientific Vivid Colors pLenti6.3/V5-GW/EmGFP Expression Control Vector

Invitrogen™ Vivid Colors™ pLenti6.3/V5-GW/EmGFP Expression Control Vector

The Vivid Colors™ pLenti6.3/V5-GW/EmGFP Expression Control Vector is a ViraPower™ HiPerform™ positive control lentiviral vector containing Emerald Green Fluorescent Protein (EmGFP).
It is designed for use with the ViraPower™ HiPerform™ Lentiviral Expression Systems as a positive control to enable detection of high EmGFP fluorescence following transfection in 293FT cells, as a titer control to produce EmGFP-expressing lentivirus stock, and as a transduction control in both dividing and non-dividing mammalian cells.

The vector features:

• CMV promoter for constitutive expression of EmGFP
• SV40 promoter for driving expression of the blasticidin resistance marker
• Woodchuck Posttranscriptional Regulatory Element (WPRE) and central Polypurine Tract (cPPT) sequences from HIV-1 integrase gene, providing at least a 4-fold increase in EmGFP expression compared to vectors without these elements

This control vector is not designed for generating EmGFP fusion proteins and does not express the V5 epitope.

Advantages

• Lentivirus-based expression of EmGFP in dividing and non-dividing mammalian cells
• Quick positive control for transfection and lentiviral production
• Useful as a titer control for determining lentivirus titer

Key Features

• Constitutive expression with CMV promoter
• High-level expression of EmGFP without V5 epitope
• WPRE and cPPT sequences enhance expression up to 4-fold
• Blasticidin selection marker for stable selection

Kit Includes

• pLenti6.3/V5-GW/EmGFP Expression Control Vector

Related Products

• ViraPower™ HiPerform™ Lentiviral TOPO™ Expression Kit (Cat. No. K531000)
• ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit (K533000)
• ViraPower™ HiPerform™ T-Rex™ Gateway™ Expression System (A11141)
• ViraPower™ HiPerform™ Promoterless Gateway™ Expression System (A11145)

Specifications