Thermo Fisher Scientific DAGLB Polyclonal Antibody
DAGLB 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal antibody로, Western blot 및 Immunocytochemistry에 적합. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관. 인간 반응성, 2-AG 및 지방산 대사 연구에 유용.
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Thermo Fisher Scientific DAGLB Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human DAGLB. Recombinant protein control fragment (Product #RP-105476) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2664114 |
Product Specific Information
Immunogen sequence:
LVALDHRKESV VVAVRGTMSL QDVLTDLSAE SEVLDVECEV QDRLAHKGIS QAARYVYQRL INDGILSQAF SIA
Highest antigen sequence identity to the following orthologs:
- Mouse: 78%
- Rat: 80%
Target Information
Lipase that catalyzes the hydrolysis of arachidonic acid (AA)-esterified diacylglycerols (DAGs) to produce the principal endocannabinoid, 2-arachidonoylglycerol (2-AG), which can be further cleaved by downstream enzymes to release arachidonic acid (AA) for cyclooxygenase (COX)-mediated eicosanoid production (PubMed:14610053).
Preferentially hydrolyzes DAGs at the sn-1 position in a calcium-dependent manner and has negligible activity against other lipids including monoacylglycerols and phospholipids (PubMed:14610053).
Plays a key role in the regulation of 2-AG and AA pools utilized by COX1/2 to generate lipid mediators of macrophage and microglia inflammatory responses.
Functions also as a polyunsaturated fatty acids-specific triacylglycerol lipase in macrophages. Plays an important role to support the metabolic and signaling demands of macrophages.
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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