Thermo Fisher Scientific BRF1 Polyclonal Antibody
BRF1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. 다양한 응용 분야(WB, IHC, ICC, IP)에 사용 가능. 항원 친화 크로마토그래피로 정제되어 높은 순도(>95%) 확보. 인간, 생쥐, 랫트 반응성. 연구용으로만 사용.
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Thermo Fisher Scientific BRF1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | - |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 220–270 of human TFIIIB90-1 (BRF1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2553482 |
Product Specific Information
- Detects endogenous protein at a molecular weight of 74 kDa.
- Purity >95% by SDS-PAGE.
Target Information
RNA polymerase III synthesizes tRNA, 5S rRNA, 7SL RNA, and U6 snRNA, and is overexpressed in many transformed cell lines and tumors. To maintain rapid growth, cells require high levels of Pol III–transcribed RNA, which depends on TFIIIB and TFIIIC2 transcription factor complexes.
TFIIIC2 is composed of five subunits (TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90, and TFIIIC63) that are overexpressed in malignant cells such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may play a key role in deregulated cell growth.
The TFIIIB complex includes TBP, TFIIB-related factor 1 (TFIIIB90, BRF1), and TFIIIB, which are upregulated in transformed cells. In many carcinomas, tumor suppressors RB and p53 are inactivated, affecting their ability to regulate TFIIIB function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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