Thermo Fisher Scientific PSMD14 Recombinant Rabbit Monoclonal Antibody (ARC1655)
PSMD14 단백질을 타깃으로 하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체입니다. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합하며, 높은 특이성과 재현성을 제공합니다. HEK293 발현 시스템 기반으로 생산되어 연구용으로 안정적입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1655 |
| Immunogen | Recombinant fusion protein containing amino acids 112–210 of human PSMD14 (UniProt ID: O00487) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849718 |
Product Specific Information
Immunogen sequence:
YHSHPGFGCW LSGVDINTQQ SFEALSERAV AVVVDPIQSV KGKVVIDAFR LINANMMVLG HEPRQTTSNL GHLNKPSIQA LIHGLNRHYY SITINYRKN
Target Information
The 26S proteasome is responsible for most intracellular proteolysis in eukaryotes. POH1 (also known as Rpn11 in yeast) subunit of the 19S complex is responsible for substrate deubiquitination during proteasomal degradation. POH1 appears to be a Zn²⁺-dependent protease, unlike other known deubiquitinating enzymes (DUBs) that are cysteine proteases. POH1 was first identified as the human functional homolog of the S. pombe pad1 gene, which confers moderate resistance to chemotherapeutic drugs and ultraviolet light when transiently overproduced in mammalian cells. Poh1-induced drug resistance is mediated through AP-1 transcription factors.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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