Thermo Fisher Scientific PSMD14 Recombinant Rabbit Monoclonal Antibody (ARC1655)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:6,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:2,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC1655

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 112-210 of human PSMD14 (O00487) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PSMD14,uniProtId:O00487-1,ncbiNodeId:9606,antigenRange:112-210,antigenLength:310,antigenImageFileName:MA5-35818_PSMD14_O00487-1_Rabbit.svg,antigenImageFileNamePDP:MA5-35818_PSMD14_O00487-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849718

Product Specific Information

Immunogen sequence: YHSHPGFGCW LSGVDINTQQ SFEALSERAV AVVVDPIQSV KGKVVIDAFR LINANMMVLG HEPRQTTSNL GHLNKPSIQA LIHGLNRHYY SITINYRKN

Target Information

The 26S proteasome is responsible for most intracellular proteolysis in eukaryotes. POH1 (also known as Rpn11 in yeast) subunit of the 19S complex is responsible for substrate deubiquitination during proteasomal degradation. POH1 appears to be a Zn21-dependent protease, unlike all other known deubiquitinating enzymes (DUBs) that are cysteine proteases. POH1 was first identified as the human functional homolog of the S. pombe pad1 gene, which confers moderate resistance to chemotherapeutic drugs, and also to ultraviolet light when transiently overproduced in mammalian cells. Poh1-induced drug resistance is mediated through AP-1 transcription factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.