Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 680

Applications and Tested Dilutions

Product Specifications

Additional Available Formats

• Alexa Fluor 488 (5 nm, 10 nm colloidal gold)
• AP, Biotin, DyLight 350/405/488/550/594/633/650/800 4X PEG
• FITC, HRP, PE-Cyanine5.5, TRITC, WesternDot 625
• Custom conjugation available upon request

Product Specific Information

Product #35568 has been validated for Western blot, IF, ICC, IHC, IP, and FACS applications.
It reacts with the heavy chains of rabbit IgG and the light chains common to most rabbit immunoglobulins, but does not react with non-immunoglobulin serum proteins.
Cross-reactivity with immunoglobulins from other species may occur.

Store protected from light at 4°C.
For long-term storage, add an equal volume of glycerol (final 50%) and store at -20°C.
DyLight 680 Amax = 682 nm; Emax = 715 nm. Mole Dye/Mole Protein Ratio is lot-dependent.

Target Information

Anti-Rabbit secondary antibodies are affinity-purified with high specificity for rabbit immunoglobulins.
They are used for detection, sorting, or purification of rabbit antibodies.
Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification.
Typically generated by immunizing the host with pooled rabbit IgG and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) for optimal performance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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