Thermo Fisher Scientific 5-Carboxylcytosine Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

1:200-1:1,000

-

Immunoprecipitation (IP)

4 µg

-

Dot blot (DB)

1:500-1:1,000

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

5-Carboxylcytosine nucleotide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

RRID

AB_2902366

Target Information

5-carboxylcytosine is the final oxidized derivative of 5-methylcytosine, a type of DNA methylation. 5-methylcytosine is oxidized to 5-hydroxymethylcytosine which is then oxidized to 5-formylcytosine and later to 5-carboxylcytosine. These oxidations are catalyzed by the Ten-Eleven Translocation (TET) enzyme family. 5-carboxylcytosine has been shown to cause reduced fidelity of RNA Polymerase II. Decreased levels of 5-hydroxymethylcytosine has been found in some cancers.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.