Thermo Fisher Scientific H3K9acK14ac Polyclonal Antibody
H3K9acK14ac 폴리클로날 항체로, 인간 및 생쥐 시료에서 히스톤 H3 아세틸화 변형 검출에 적합합니다. WB, ICC, ELISA, ChIP, ChIP-Seq 등 다양한 응용에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| ELISA | 1:100 |
| ChIP assay (ChIP) | 1 µg |
| ChIP-sequencing (ChIP-Seq) | 1 µg |
| Peptide Array (Array) | 1:2,000 |
| Dot blot (DB) | 1:20,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | H3K9acK14ac synthetic peptide |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.81 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide, 0.05% ProClin 300 |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2902365 |
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translationally, histones are modified in various ways to either directly change the chromatin structure or allow for the binding of specific transcription factors.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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