Thermo Fisher Scientific Nur77 Monoclonal Antibody (12.14), Alexa Fluor 488, eBioscience
Mouse Nur77 단백질을 인식하는 Alexa Fluor 488 결합 단클론 항체로, 세포내 염색 및 유세포분석(Flow Cytometry)에 최적화되어 있습니다. 488 nm 여기, 519 nm 방출 특성을 가지며, 4°C 암소 보관이 권장됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 6 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 12.14 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574428 |
Available Formats
Product Specific Information
Description:
This 12.14 monoclonal antibody reacts with mouse Nur77 (also known as NR4A1, TR3, NGFI-B, or NAK1), an inducible orphan nuclear receptor. Nur77 is expressed in thymocytes and T cell lines, promotes apoptosis, and plays a role in thymocyte negative selection. It is also involved in steroid biosynthesis in Leydig cells and dopamine signaling. Additionally, Nur77 interacts with FoxP3 in regulatory T cells, though results with this antibody do not correlate with that observation.
Applications Reported:
This antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested:
Tested by intracellular staining and flow cytometry of stimulated mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00).
Recommended use ≤ 1 µg per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test (empirically determined).
Optimize antibody titration for best performance.
Spectral Properties:
Excitation: 488 nm
Emission: 519 nm
Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
NR4A1 belongs to the steroid-thyroid hormone-retinoid receptor superfamily. It is expressed in multiple tissues including brain, adrenal, thyroid, liver, testis, ovary, thymus, muscle, lung, and prostate. Expression is induced by stress stimuli and growth factors. The protein functions as a nuclear transcription factor mediating apoptosis and hypothalamic-pituitary signaling. Translocation from nucleus to mitochondria triggers apoptosis. Three transcript variants produce two isoforms, with variant 3 (TRC beta) differing at the C-terminus. RXR is a known partner of NR4A1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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