Thermo Fisher Scientific TAF5 Polyclonal Antibody
TAF5 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot, IHC, IP 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제됨. 인간 시료에 반응하며 4°C에서 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 350–400 of Human TAF5 (TAF5 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 100 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Phosphate/Tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
TFIID is a general transcription factor that initiates preinitiation complex assembly through direct interaction with the TATA promoter element. It is a multisubunit complex consisting of a small TATA-binding polypeptide and other TATA-binding protein (TBP)-associated factors (TAFs).
TAF II p100 (also known as TAF5 or TAFII100) is the third largest subunit of human TFIID. It contains six WD40 repeats at the C-terminus and an N-terminus capable of forming a flexible dimer. TAF II p100 plays an important role in forming the scaffold crucial for TFIID complex assembly and may help stabilize TAF interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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