
Thermo Fisher Scientific Rabbit anti-Human IgA2 Recombinant Secondary Antibody, Biotin
Recombinant rabbit monoclonal anti-human IgA2 항체로, Biotin으로 표지된 2차 항체입니다. 높은 특이성과 재현성을 제공하며, IHC, ICC/IF, ELISA 등에 적합합니다. Protein A 정제 및 동결보존 가능하며 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | 0.1–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–2 µg/mL |
| ELISA | 50–200 ng/well |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Secondary Antibody |
| Clone | RM125 |
| Immunogen | IgA2 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2744973 |
| Target | IgA2 |
| Antibody Form | Whole Antibody |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems are developed by cloning specific antibody DNA sequences from immunoreactive rabbits, followed by screening of individual clones to select optimal candidates for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Anti-Human secondary antibodies are affinity-purified and exhibit well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of target molecules.
Secondary antibodies offer:
- Versatility across detection systems (e.g., HRP, AP, fluorescence)
- Increased sensitivity through signal amplification
Typically, they are generated by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to ensure high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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