Thermo Fisher Scientific Phospho-SHC (Tyr427) Polyclonal Antibody
Phospho-SHC (Tyr427) 폴리클로날 항체로 인간 시료에 반응하며, Western blot, IHC, ICC/IF 등에 사용 가능. 고순도 친화 크로마토그래피 정제, 액상 형태로 제공되며, 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:100–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 427 (P-S-Y(p)-V-N) derived from Human Shc1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2554346 |
Product Specific Information
- Suggested positive control for Western blot: 293 cells
- Suggested positive control for IHC: human breast carcinoma
- Suggested positive control for ICC/IF: HeLa cells
Target Information
Skp2 (S-phase Kinase-associated Protein 2) is a member of the F-box protein family that interacts with the Cyclin A-Cdk2 complex and is essential for G1-S transition. Skp2 is involved in ubiquitination and degradation of p27 in mammalian cells. Expressed in tonsil and placenta under normal conditions, and widely in tumor tissues such as colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers. Research suggests Skp2 plays a role in oncogenesis, particularly in lymphomas and breast cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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