Thermo Fisher Scientific Phospho-Desmin (Thr76, Thr77) Polyclonal Antibody
인산화된 Desmin(Thr76, Thr77)을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 IHC(Paraffin) 분석에 적합합니다. 인간, 생쥐, 랫트 시료에 반응하며, 항원 친화 크로마토그래피로 정제되었습니다. 세포질 및 막 위치 단백질 검출에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide SRL G-*T-*T-RTP corresponding to the N-terminus with a dual phosphorylated threonine (Thr76/Thr77) of human Desmin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 25 mM Tris with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: Cardiac Myocyte
- Cellular location: Cytoplasmic and membrane
Target Information
Desmin (approximately 53 kDa) exhibits a high degree of tissue specificity, being predominantly expressed in all types of muscle cells (cardiac, skeletal, and smooth muscle). Its expression is stage- and tissue-specific, induced during terminal development such as skeletal muscle differentiation.
In skeletal and cardiac muscle cells, desmin is localized in the Z-disk region and at the intercalated disk. Expression in smooth muscle is more heterogeneous. Coexpression of vimentin and desmin has been observed in muscle-derived tumors such as rhabdomyosarcomas and leiomyosarcomas.
During myocardial dysfunction, dramatic changes in the distribution of desmin have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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