Thermo Fisher Scientific Ubiquitin Monoclonal Antibody (FK1), PerCP
Ubiquitin 단백질의 폴리유비퀴틴 사슬을 특이적으로 검출하는 PerCP 결합 단클론 항체. Western blot, IHC, ICC/IF, ELISA에 사용 가능. 마우스 IgM 항체로 4°C 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | All, Human |
| Host / Isotype | Mouse / IgM |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FK1 |
| Immunogen | Ubiquitin conjugated lysozyme |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Size-exclusion (Gel filtration) |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931781 |
Additional Formats
Product Specific Information
A 1:5000 dilution of MA5-45327 was sufficient for detection of ubiquitin conjugates in 2.5 µg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects polyubiquitinated proteins and ubiquitin chains. Does not detect monoubiquitinated proteins nor free ubiquitin.
Target Information
Ubiquitin is a highly conserved protein that plays a major role in targeting cellular proteins for degradation by the 26S proteasome. It is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. The gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed to generate free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S ribosomal subunit, contains C4-type zinc finger domains, and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. Similarly, ribosomal proteins L40 and S30 are synthesized as fusion proteins with ubiquitin or ubiquitin-like proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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