Thermo Fisher Scientific GRP78 Monoclonal Antibody (3G12-1G11), FITC
GRP78 단백질을 인식하는 FITC 결합 단클론 항체로, Western blot, ICC/IF, ELISA에 사용 가능. 인간, 마우스, 랫트 반응성. 단백질 접힘 및 스트레스 반응 연구에 적합. 4°C 암소 보관, Protein G 정제.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3G12-1G11 |
| Immunogen | Full-length recombinant rat GRP78 |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931777 |
Additional Formats
- APC (Cat. No. MA5-45322)
- PerCP (Cat. No. MA5-45324)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45323 was sufficient for detection of GRP78 in 20 µg of HEK-293 lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 78 kDa.
Target Information
GRP78 is a 78 kDa glucose-regulated protein belonging to the HSP70 family. It resides in the endoplasmic reticulum (ER) and associates with newly synthesized secretory and membrane proteins. GRP78 also interacts with mutant or misfolded proteins, targeting them for degradation.
The conserved C-terminal sequence Lys-Asp-Glu-Leu (KDEL) enables ER retention via the KDEL receptor. GRP78 assists in protein folding and degradation of misfolded proteins through interaction with DNAJC10, maintaining cellular homeostasis and preventing apoptosis.
GRP78 levels serve as biomarkers for hypoglycemia and have neuroprotective roles under oxidative stress. Reduced GRP78 expression is observed in Alzheimer’s disease and is linked to PS1 gene mutations. Its induction is also associated with antitumor drug resistance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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