Thermo Fisher Scientific Ubiquitin Monoclonal Antibody (FK2), FITC
Ubiquitin 단백질 및 유비퀴틴화된 단백질 검출용 FITC 결합 단클론 항체. Western blot, IHC, ICC/IF, ELISA, IP 등에 사용 가능. 모든 종에 반응하며, 형광 검출에 적합. 단백질 A 정제, 4°C 암소 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| ELISA | 1:1,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | All, Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | FK2 |
| Immunogen | Ubiquitin conjugated lysozyme |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage buffer | 9.09 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage conditions | 4°C, store in dark |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931783 |
Additional Formats
- APC (Cat. No. MA5-45328)
- PerCP (Cat. No. MA5-45330)
- Custom conjugation available upon request
Product Specific Information
A 1:5000 dilution of MA5-45329 was sufficient for detection of ubiquitin conjugates in 2.5 µg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects ubiquitinated proteins and ubiquitin chains. Does not detect free ubiquitin.
Target Information
Ubiquitin is a highly conserved protein involved in targeting cellular proteins for degradation by the 26S proteasome. It is synthesized as a precursor protein, either as polyubiquitin chains or as a single ubiquitin fused to another protein.
This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed, it is processed to generate free ubiquitin and ribosomal protein S27a.
Ribosomal protein S27a is part of the 40S ribosomal subunit, contains C4-type zinc finger domains, and is localized in the cytoplasm. Pseudogenes derived from this gene are present in the genome.
Similarly, ribosomal proteins L40 and S30 are also synthesized as fusion proteins with ubiquitin or ubiquitin-like proteins. Multiple alternatively spliced transcript variants exist but encode the same proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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